Fig. (1) The level of apoptosis of human fibroblasts, resting or activated with LPS in the absence of simvastatin. The level of apoptosis is among 19% to 21%.The level of apoptosis of resting or activated with lipopolysaccaride (LPS; 3µg/ml) human fibroblasts in the medium of synovial culture tissue not treated with simvastatin. Apoptosis was analyzed by two-dimensional flow cytometry using annexin V and propidium iodide according to the manufacturer’s instructions (Biotest). Events accumulating at the top and bottom right-hand quadrates simulate apoptotic cells.