Histological Examination of Collagen and Proteoglycan Changes in Osteoarthritic Menisci

Table 3: Grades of Safranin-O and Alcian Blue Staining

	Normal 12F	Normal 39F	Normal 43M	OA 42M	OA 49F	OA 54F	OA 55M	OA 58F	OA 65F	OA 66F	OA 70F
Safranin-O
Grade_T	0	2	0	3	3	5	4	2	5	4	3
Grade_H	2	2	0	3	3	5	4	3	5	5	4
Alcian Blue
Grade_T	0	1	0	3	4	4	3	3	5	4	4
Grade_H	1	2	0	3	3	5	4	3	5	4	5

Grade_T – Transverse section grade; Grade_H – Horizontal section grade; Ages are listed in years; M = male, F = female. The difference of safranin-O staining grades between OA group and normal control group was statistically significant (p = 0.018). The difference of alcian blue staining grades between OA group and normal control group was statistically significant (p = 0.014).