Fig. (3) Verification of microarray results using quantitative real-time PCR analysis for NANOG, OCT4, TDGF1, SOX2, GAL, IGFB7, COL5A2, and COL11A1 expression in feeder-cultured hESCs and MFG-hESCs. Data represent averages ± SD (* indicates P < 0.05). Abbreviations: hESCs, human embryonic stem cells; MFG-hESCs, matrix-free growth adapted human embryonic stem cells.