Fig. (3) MSC-CM restores glucose-stimulated insulin secretion in BRIN-BD11 cells following cytokine challenge. BRIN-BD11 cells were exposed to 1 µg/ml IFN-γ, 100 ng/ml TNF-α, and 100 ng/ml IL-1β for 24h prior to exposure to rising concentrations of D-Glucose (1.1, 5.6, 16.7 mM) in the presence or absence of MSC-CM. Insulin secretion was measured by ELISA and data presented according to insulin concentration or insulin concentration as a function of protein content. Data are presented as mean ± standard deviation (SD). n=4 with all experiments assayed in duplicate. *P<0.05, **P<0.01, and ***P<0.001 compared with corresponding treatments in the absence of MSC-CM. MSC-CM, Mesenchymal Stem Cell Conditioned Media; IFN-γ, Interferon gamma; TNF-α, Tumour Necrosis Factor alpha; IL-1β, Interleukin-1 beta.