Fig. (6) Effects of glucose on CA-induced NRF2 activation in MCF-7 cells. (A): MCF-7 cells were transfected with pAREx8-TATA, and further incubated in the medium containing various concentrations (1.0 or 4.5 g/L) of D-glucose for 24 hours (Naive). (B): MCF-7 cells were transfected with pAREx8-TATA, and further incubated in the medium containing various concentrations (1.0 or 4.5 g/L) of D-glucose in the presence of 64 μM CA for 24 hours (CA treatment). The luciferase activity was determined, and data were expressed as relative light units (RLU: firefly luciferase activity divided by Renilla luciferase activity). All experiments were performed in triplicate, and values are indicated as mean ± S.D. Statistically significant differences are indicated by an asterisk (**p < 0.01, *p < 0.05 by Student’s t-test).