Table 2: Mutations and their percentages identified by comparing to GFPm gene.

Amplicon	Location*	Reference nucleotide (GFPm)	Variant nucleotide (EGFP)	Total Depth	Variant Frequency
>Rec1-EGFP	21	A	G	7182	37%
>Rec1-EGFP	30	T	C	7093	37%
>Rec1-EGFP	33	C	G	7086	37%
>Rec1-EGFP	39	C	G	7040	37%
>Rec1-EGFP	48	C	G	6797	35%
>Rec1-EGFP	51	G	C	6731	34%
>Rec1-EGFP	54	A	G	6716	34%
>Rec1-EGFP	60	T	C	6683	34%
>Rec1-EGFP	66	T	C	6315	31%
>Rec1-EGFP	117	A	C	6437	41%
>Rec1-EGFP	123	A	C	6425	41%
>Rec1-EGFP	129	C	G	6416	39%
>Rec1-EGFP	138	A	G	6403	39%
>Rec1-EGFP	153	T	C	6343	38%
>Rec1-EGFP	165	A	C	6300	36%
>Rec2-EGFP	289	C	T	15531	20%
>Rec3-EGFP	456	C	T	15566	29%
>Rec3-EGFP	474	A	G	14345	37%
>Rec3-EGFP	480	T	C	14178	39%
>Rec3-EGFP	492	C	G	13736	43%
>Rec3-EGFP	505	A	C	13035	45%
>Rec3-EGFP	507	A	C	13023	45%
>Rec3-EGFP	514	A	G	12684	39%
>Rec3-EGFP	607	T	A	8032	37%
>Rec3-EGFP	608	C	G	8029	37%
>Rec3-EGFP	618	T	C	8407	36%
>Rec3-EGFP	633	T	C	8606	35%
>Rec3-EGFP	641	A	C	8598	36%
>Rec3-EGFP	642	A	G	8598	36%
>Rec3-EGFP	646	A	C	8711	35%
>Rec3-EGFP	648	A	C	8706	35%
>Rec3-EGFP	651	C	T	8698	35%
>Rec3-EGFP	672	T	C	8616	100%
>Rec3-EGFP	681	T	C	8601	100%
>Rec3-EGFP	684	T	C	8591	100%
>Rec3-EGFP	693	A	T	8643	100%
>Rec3-EGFP	695	A	T	8648	100%
>Rec3-EGFP	696	T	C	8646	100%
* The locations of mutations are determined according to EGFP sequence. Mutations in the first variable region (21bp-165bp) are shown in regular. The C289T mutation is highlighted in bold and underlined. Mutations in the second variable region (456bp-651bp) are marked in italic. Mutations in the third variable region (672bp-696bp) are underlined.