Fig. (2) Effect of pre-incubation of leaf extracts with HSV-1, HSV-2 and VZV on virus infectivity. (A) 1000 µg/ml of C. fragrans ethanol leaf extract, (B) 1000 µg/ml of C. fragrans aquatic leaf extracts and (C) 100 µg/ml of S. chinensis aquatic leaf extracts were incubated with the infecting virus (5m.o.i.) at 4°C for different periods of time (15, 30 and 45 minutes). Every mixture was diluted 104 times with fresh medium and the cells were infected with the diluted mixture. PFU were evaluated by the standard plaque assay and presented as means ± SD (n=5).