Table 1.: Summary of Main Molecular Testing Techniques for Viruses

Technique Format Examples of viruses

NON AMPLIFIED NUCLEIC ACID PROBES Liquid-phase HPV, CMV
Solid-phase
In situ hybridization
AMPLIFIED NUCLEIC ACID TECHNIQUES
Signal amplification techniques

bDNA assays

HCV, HBV, HIV
Hybrid capture assays HPV, CMV
Target amplification techniques PCR techniques Most of viruses
RT-PCR RNA virus (HCV, HIV)
Nested PCR Herpesviruses
Multiplex PCR Herpesviruses, respiratory viruses
Real-time PCR Most of viruses
Transcriptional-based amplification methods HCV (TMA-based), HIV (TMAbased, NASBA), CMV (NASBA) Enterovirus (NASBA), RSV (NASBA)
Strand displacement amplification HIV
LAMP Influenza A and B, CMV, HSV, VZV, BK virus, HPV
HDA HIV-1, HSV 1 and 2
Probe amplification techniques Ligase chain reaction HCV, HPV
Cycling probe technology
Cleavase-invader technology
MICROARRAYS DNA microarrays Respiratory viruses, HCV, HPV, HIV, CNS infection viruses
Multiplexed microsphere-based array HIV, HCV, HSV

HPV: human papillomavirus; CMV: cytomegalovirus; HCV: hepatitis C virus; HBV: hepatitis B virus; HIV: human immunodeficiency virus; TMA: transcription-mediated amplification; NASBA: nucleic acid sequence-based amplification; RSV: respiratory syncytial virus; DNA: deoxyribonucleic acid; RNA: ribonucleic acid; LAMP: loop-mediated isothermal amplification; HDA: helicase-dependent amplification.