Table 2.: Main Features of Commercial EIAs (Adapted from Kim et al. J Clin Microbiol 2008; 46: 3919-23

Manufacturer Analyzers Core NS3 NS4 NS5 Assay Format Solid Phase Labeled Substance Sample Vol Time of Reaction Gray Zone Signal-to-Cut-Off Ratio Predictive of a True Positive ≥ 95% Time
Roche Elecsys 2010 Cobas e 411 Cobas e 601 Modular analiytics E170 Present Present Present Absent CLIA Magnetic particle Ruthenium complex 40 18 0.9-1.0 ≥ 3.8
Abbott Laboratories Architect i2000SR system HCr43 HCr43 c100-3 Absent CMIA Paramagnetic particle Acridinium 20 29 Not indicated ≥ 5.0
Ortho-Clinical Diagnostics Vitros EciQ Vitros 3600 system Immunodiagnostic System c22-3 c200 c200 Present CLIA Well Luminal derivate 20 56 0.9-1.0 ≥ 8.0
BioRad UniCel DxI 800 Access or Access 2 or UniCel (Beckman Coulter) Present Present Present Absent CLIA Paramagnetic particle Lumi-Phos 530 25 55 0.9-1.0 ≥ 10.0
Abbott Laboratories AxSYM HCr43 C200 C100-3 Present MEIA Paramagnetic particle 4- Methylumbelliferyl Phosphate 33 30 0.8 - 1.2 ≥10.0
Siemens Advia centaur C22-3 Present c200 Present CLIA Magnetic particle Acridinium 10 58 0-9-1.0 ≥ 11.0
BioMerieux Vidas HCr43 Present C100-3 Absent ELFA Well 4- Methylumbelliferyl Phosphate 100 40 Not indicated Not available
Abbott Laboratories Prism HCr43 HCr43 c100-3 Present CLIA Well Acridinium 50 54 Not indicated Not available
DiaSorint Eti-MAX 3000 HCr43 HCr43 c100-3 Present ELISA Well 3,3’,5,5’- tetramethylbenzidine 20 90 Not indicated Not available
Ortho-Clinical Diagnostics Microtite plate c22-3 c200 c200 Present ELISA Well 3,3’,5,5’- tetramethylbenzidine 20 90 Not indicated ≥ 3.8
Abbott Laboratories Semi-automated (IMx) Present Present Present Present ELISA Well 3,3’,5,5’- tetramethylbenzidine 20 90 Not indicated ≥ 3.8

ELISA: Enzyme-linked Immunosorbent Assay; CLIA: Chemiluminescence Immunoassay; MEIA: Microparticle capture Enzyme ImmunoAssay Ortho-Clinical Diagnostics, Buckinghamshire, Germany; Roche Diagnostics GmbH, Mannheim Germany; Abbott Laboratories, Wiesbaden, Germany; Siemens Healthcare Diagnostics, Deerfield, IL; BioMerieux Clinical Diagnostics, France; Bio-Rad, Marnes-la-Coquette, France.