Manufacturer |
Analyzers |
Core |
NS3 |
NS4 |
NS5 |
Assay Format |
Solid Phase |
Labeled Substance |
Sample Vol |
Time of Reaction |
Gray Zone |
Signal-to-Cut-Off Ratio Predictive of a True Positive ≥ 95% Time |
Roche |
Elecsys 2010
Cobas e 411
Cobas e 601
Modular
analiytics E170 |
Present |
Present |
Present |
Absent |
CLIA |
Magnetic
particle |
Ruthenium
complex |
40 |
18 |
0.9-1.0 |
≥ 3.8 |
Abbott
Laboratories |
Architect
i2000SR system |
HCr43 |
HCr43 |
c100-3 |
Absent |
CMIA |
Paramagnetic
particle |
Acridinium |
20 |
29 |
Not
indicated |
≥ 5.0 |
Ortho-Clinical
Diagnostics |
Vitros EciQ
Vitros 3600
system
Immunodiagnostic
System |
c22-3 |
c200 |
c200 |
Present |
CLIA |
Well |
Luminal
derivate |
20 |
56 |
0.9-1.0 |
≥ 8.0 |
BioRad |
UniCel
DxI 800
Access or
Access 2 or
UniCel
(Beckman Coulter) |
Present |
Present |
Present |
Absent |
CLIA |
Paramagnetic
particle |
Lumi-Phos
530 |
25 |
55 |
0.9-1.0 |
≥ 10.0 |
Abbott
Laboratories |
AxSYM |
HCr43 |
C200 |
C100-3 |
Present |
MEIA |
Paramagnetic
particle |
4-
Methylumbelliferyl
Phosphate |
33 |
30 |
0.8 - 1.2 |
≥10.0 |
Siemens |
Advia centaur |
C22-3 |
Present |
c200 |
Present |
CLIA |
Magnetic
particle |
Acridinium |
10 |
58 |
0-9-1.0 |
≥ 11.0 |
BioMerieux |
Vidas |
HCr43 |
Present |
C100-3 |
Absent |
ELFA |
Well |
4-
Methylumbelliferyl
Phosphate |
100 |
40 |
Not
indicated |
Not
available |
Abbott
Laboratories |
Prism |
HCr43 |
HCr43 |
c100-3 |
Present |
CLIA |
Well |
Acridinium |
50 |
54 |
Not
indicated |
Not
available |
DiaSorint |
Eti-MAX
3000 |
HCr43 |
HCr43 |
c100-3 |
Present |
ELISA |
Well |
3,3’,5,5’-
tetramethylbenzidine |
20 |
90 |
Not
indicated |
Not
available |
Ortho-Clinical
Diagnostics |
Microtite
plate |
c22-3 |
c200 |
c200 |
Present |
ELISA |
Well |
3,3’,5,5’-
tetramethylbenzidine |
20 |
90 |
Not
indicated |
≥ 3.8 |
Abbott
Laboratories |
Semi-automated
(IMx) |
Present |
Present |
Present |
Present |
ELISA |
Well |
3,3’,5,5’-
tetramethylbenzidine |
20 |
90 |
Not
indicated |
≥ 3.8 |
ELISA: Enzyme-linked Immunosorbent Assay; CLIA: Chemiluminescence Immunoassay; MEIA: Microparticle capture Enzyme ImmunoAssay Ortho-Clinical Diagnostics,
Buckinghamshire, Germany; Roche Diagnostics GmbH, Mannheim Germany; Abbott Laboratories, Wiesbaden, Germany; Siemens Healthcare Diagnostics, Deerfield, IL;
BioMerieux Clinical Diagnostics, France; Bio-Rad, Marnes-la-Coquette, France.