Fig. (3) Evaluation of large-scale extracts and fractions. (a) Each large scale extract and the fractions that originated from it were evaluated for cytotoxicity using the MTT assay. Controls for DMSO concentration (solvent), complete cell death (negative) and no treatment (positive) were also performed. The % cellular survival was determined by comparison with the solvent control. (b) Inhibition of large-scale extracts and fractions was determined by infecting A3.01 cells with HIV-1_LAV in the presence of the large scale extracts and fractions at 8 µg/mL (except for DB, 2 µg/mL, and DB-2, 0.25, 0.5, 1 µg/mL). Controls for DMSO concentration present in extract wells (DMSO), positive inhibition of viral replication (10 µM and 20 µM AZT), and absence of extract (LAV) and virus (mock) were included. Virus levels in culture supernatant were determined by RT assay.