Fig. (5) Efficacy of isolated individual compounds. (a) MTT assays were performed to determine the cytotoxicity of the compounds against A3.01 cells. Controls for DMSO concentration (solvent), as well as positive (cells alone) and negative (addition of 100% DMSO) controls were performed. The % of cellular survival was determined by comparison with the solvent control. (b) The effect of the individual compounds DK, DL, DM, DN and DP on HIV-1 replication was determined by infecting A3.01 cells with HIV-1_LAV in the presence of the compounds. DK, DM, DN and DP were tested at 1.5 µg/mL, while DL was tested at 0.5 µg/mL. Controls for DMSO concentration present in extract wells (DMSO), positive inhibition of viral replication (10 µM and 20 µM AZT), and absence of extract (LAV) and virus (mock) were included. Virus levels in culture supernatant were determined by RT assay.