Exploration of New Sites in Adenovirus Hexon for Foreign Peptides Insertion

Table 1: Synthetic oligonucleotides used for PCR to insert the sequence corresponding to the PRRSV epitope B into HVRs of Ad5 hexon.

Name	Sequence^a
BL7F1	TTTACAACCTCCTAGGCAGCAATACAGAGACTCTTACCAA
BL7R	TGTAGATGGGAAGAACCTAAAATCACACCTCCCAGTGGAA
BL8F1	TTTACAACCTCCTAGGCAGCGGATGGGAAAAAGATGCTAC
BL8R	TGTAGATGGGAAGAACCTAAATTTTCCTGACCTGTTTTAG
BL9F1	TTTACAACCTCCTAGGCAGCTTTTCAGATAAAAATGAA
BL9R	TGTAGATGGGAAGAACCTAATTCTGTAGCATCTTTTT
BLF2	TTAGGTTCTTCCCATCTACAGCTGATTTACAACCTCCTAGGCAGC
Nde-F	CGGCATCAGAGCAGATTGTA
Nhe-R	ATGCGGTCCTTGTAGCTCTC

^a Letters representing the sequences encoding the PRRSV epitope and flanking LGS amino acid residues are in bold.